When it was clear that crystal meth was a large community driver for HIV infections among gay men, Tweaker.org was created as part of a social marketing campaign to help gay men understand crystal meth and how it affects their sexual risk-taking and overall health.

The website was first launched in 1997 by STOP AIDS Project and was revitalized by the Stonewall Project in 2002. Updated and redesigned in 2019, Tweaker.org is viewed by more than 980,000 unique visitors across the globe annually.